Composite

Part:BBa_K4676003:Design

Designed by: Nicholas H. Perez   Group: iGEM23_Hopkins   (2023-10-11)


Truncated Control H-Fibroin Transcriptional Unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When choosing the promoter, it was important to choose a promoter that would work in T7 strain E. coli; given that T7 Shuffle strain was specifically selected as a chassis, in order to boost disulfide bond formation between the H-Fibroin and the L-Fibroin silk proteins.


Source

See pages for each of the individual parts.

References